Jing Cui assisted with animal anesthetization, IOP measurement and. RGC cellular characteristics were correlated with functional visual field measurements. Quantitative pupillography may be useful in diagnosis and monitoring of optic neuropathies. inflammasome activation contributes to RGC death, mice were treated with 8 weeks. Ganglion cell layer (GCL) somas were manually counted, and morphological parameters of GCL soma density, size, and symmetry were calculated. There is a statistically significant association between estimated RGC count and pupillary response to stimuli. Single-cell sequencing was performed on mature and functional iPSC-RGCs at day 40 using Chromium Single Cell 3’ V3 protocols (10X Genomics). For every 0.3 log unit difference in RAPD score, there was an average decrease in estimated RGC count of 37,817 (P<0.001). We intend to identify marker genes with differential gene expression (DEG) and RGC subtypes in cultures of human-induced pluripotent stem cell (iPSC)-derived retinal ganglion cells. For analyses performed at the level of individual eyes, we used multilevel modeling to account for between-eye correlations within individuals.Įyes with higher estimated RGC counts had a larger pupil response amplitude (z-score=8.24, P<0.001), shorter latency (z=-3.17, P=0.002), faster constriction velocity (z=6.64, P<0.001), shorter time to maximum constriction (z=-1.96, P=0.049), and longer time to maximum dilation (z=6.66, P<0.001). RGC counts were estimated using empirical formulas that combine estimates from both functional (eccentricity and sensitivity measured by standard automated perimetry) and structural (retinal nerve fiber layer thickness) tests. The magnitude of a relative afferent pupillary defect (RAPD) was defined as the log of the ratio of the amplitude between 2 eyes multiplied by 10. The specificity of RGC labelling with AAV vectors or comparing promoters has not been previously quantified with RGC-specific immunohistochemistry or markers. The amplitude of pupil constriction was calculated as the percentage change in pupil diameter between constriction onset and peak constriction in response to controlled stimuli. Functional, morphological, and transcriptomic surveys have identified more than 40 retinal ganglion cell (RGC) types in mice. Methods: A cross-sectional study was conducted including both eyes of 103 participants from the Diagnostic Innovations in Glaucoma Study. We recorded and analyzed pupil responses using an automated binocular pupillometer in 148 patients with glaucoma (mean age 67☑1, 49% female) and 71 controls (age 60☙.6, 69% female) as part of a prospective clinical study. Purpose: To estimate retinal ganglion cell (RGC) losses associated with a relative afferent pupillary defect (RAPD) in glaucoma. To assess the relationship between pupillary light reflex and estimated retinal ganglion cell (RGC) counts in patients with glaucoma.
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